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RICE MORPHOLOGY DETERMINANT Encodes the Type II Formin FH5 and Regulates Rice Morphogenesis.

Plant Cell. 2011 Feb 9;

Authors: Zhang Z, Zhang Y, Tan H, Wang Y, Li G, Liang W, Yuan Z, Hu J, Ren H, Zhang D

Multicellular organisms contain a large number of formins; however, their physiological roles in plants remain poorly understood. Here, we reveal that formin homology 5 (FH5), a type II formin mutated in rice morphology determinant (rmd), plays a crucial role in determining rice (Oryza sativa) morphology. FH5/RMD encodes a formin-like protein consisting of an N-terminal phosphatase tensin (PTEN)-like domain, an FH1 domain, and an FH2 domain. The rmd mutants display a bending growth pattern in seedlings, are stunted as adult plants, and have aberrant inflorescence (panicle) and seed shape. Cytological analysis showed that rmd mutants have severe cell elongation defects and abnormal microtubule and microfilament arrays. FH5/RMD is ubiquitously expressed in rice tissues, and its protein localization to the chloroplast surface is mediated by the PTEN domain. Biochemical assays demonstrated that recombinant FH5 protein can nucleate actin polymerization from monomeric G-actin or actin/profilin complexes, cap the barbed end of actin filaments, and bundle actin filaments in vitro. Moreover, FH5 can directly bind to and bundle microtubules through its FH2 domain in vitro. Our findings suggest that the rice formin protein FH5 plays a critical role in determining plant morphology by regulating actin dynamics and proper spatial organization of microtubules and microfilaments.

Rice Atlas Database -- 一个水稻表达图表数据库

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虽然叫做Rice Atlas Database,不过其实并没有收入水稻所有时期组织的表达情况.主要就是苗的根和叶及其相关组织,根尖,表皮等等,没有花和茎.作为参考就行,毕竟水稻中做的芯片数据不如拟南芥那么全.

参考文献:
A transcriptome atlas of rice cell types uncovers cellular, functional and developmental...